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sds page pre cast gels  (Bio-Rad)


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    Bio-Rad sds page pre cast gels
    Analysis of SP-EVs fractions by (a) transmission electron microscopy (TEM) and (b, c) nanoparticle tracking analysis (NTA, n = 4). (d) Protein concentration (μg/μL) (n = 9) was assessed by Abs280 nm. <t>(e)</t> <t>SDS-PAGE</t> (15%) protein profile of SP-EV preparations and SP (60 μg protein/lane) (n = 3) stained with Coomassie brilliant blue. (f) Western blot analysis (60 μg protein/lane) of EV preparations and complete SP using antibodies against EV-associated proteins CD9, CD63, and HSP70, endoplasmic reticulum marker calnexin (CNX), and a non-vesicular extracellular particle apolipoprotein C-III (ApoC3) (n = 3) (full western blot images in Supplementary Figure 2). SP: seminal plasma; P1–2: pool of fractions in size-exclusion chromatography; CL: cell lysate; Mw: molecular weight marker. Different letters (a, b) above the bars indicate statistically significant differences (p < 0.05) between methods (C, P1, and P2), as determined by a linear mixed-effects model and Tukey-adjusted pairwise comparisons. A significant positive correlation was observed between particle concentration and protein concentration across samples (Spearman’s ρ = 0.747, p = 0.0009). All data are expressed as mean ± SD of four independent experiments (n = 4). Scale bar = 200 nm.
    Sds Page Pre Cast Gels, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 714 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mini+protean+tgx+stain+free+pre+cast+gel/10%25+Mini-PROTEAN+TGX+Stain-Free+Protein+Gels/pmc13067157-35-3-6
    Average 96 stars, based on 714 article reviews
    sds page pre cast gels - by Bioz Stars, 2026-09
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    Images

    1) Product Images from "Uptake Assay of Ram Seminal Plasma Extracellular Vesicles to Sperm"

    Article Title: Uptake Assay of Ram Seminal Plasma Extracellular Vesicles to Sperm

    Journal: Bio-protocol

    doi: 10.21769/BioProtoc.5653

    Analysis of SP-EVs fractions by (a) transmission electron microscopy (TEM) and (b, c) nanoparticle tracking analysis (NTA, n = 4). (d) Protein concentration (μg/μL) (n = 9) was assessed by Abs280 nm. (e) SDS-PAGE (15%) protein profile of SP-EV preparations and SP (60 μg protein/lane) (n = 3) stained with Coomassie brilliant blue. (f) Western blot analysis (60 μg protein/lane) of EV preparations and complete SP using antibodies against EV-associated proteins CD9, CD63, and HSP70, endoplasmic reticulum marker calnexin (CNX), and a non-vesicular extracellular particle apolipoprotein C-III (ApoC3) (n = 3) (full western blot images in Supplementary Figure 2). SP: seminal plasma; P1–2: pool of fractions in size-exclusion chromatography; CL: cell lysate; Mw: molecular weight marker. Different letters (a, b) above the bars indicate statistically significant differences (p < 0.05) between methods (C, P1, and P2), as determined by a linear mixed-effects model and Tukey-adjusted pairwise comparisons. A significant positive correlation was observed between particle concentration and protein concentration across samples (Spearman’s ρ = 0.747, p = 0.0009). All data are expressed as mean ± SD of four independent experiments (n = 4). Scale bar = 200 nm.
    Figure Legend Snippet: Analysis of SP-EVs fractions by (a) transmission electron microscopy (TEM) and (b, c) nanoparticle tracking analysis (NTA, n = 4). (d) Protein concentration (μg/μL) (n = 9) was assessed by Abs280 nm. (e) SDS-PAGE (15%) protein profile of SP-EV preparations and SP (60 μg protein/lane) (n = 3) stained with Coomassie brilliant blue. (f) Western blot analysis (60 μg protein/lane) of EV preparations and complete SP using antibodies against EV-associated proteins CD9, CD63, and HSP70, endoplasmic reticulum marker calnexin (CNX), and a non-vesicular extracellular particle apolipoprotein C-III (ApoC3) (n = 3) (full western blot images in Supplementary Figure 2). SP: seminal plasma; P1–2: pool of fractions in size-exclusion chromatography; CL: cell lysate; Mw: molecular weight marker. Different letters (a, b) above the bars indicate statistically significant differences (p < 0.05) between methods (C, P1, and P2), as determined by a linear mixed-effects model and Tukey-adjusted pairwise comparisons. A significant positive correlation was observed between particle concentration and protein concentration across samples (Spearman’s ρ = 0.747, p = 0.0009). All data are expressed as mean ± SD of four independent experiments (n = 4). Scale bar = 200 nm.

    Techniques Used: Transmission Assay, Electron Microscopy, Protein Concentration, SDS Page, Staining, Western Blot, Marker, Clinical Proteomics, Size-exclusion Chromatography, Molecular Weight, Concentration Assay

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    Article Snippet: .. Solutions were incubated on ice for 20 min and then pelleted at 17,000 × g for 5 min. Pellets were rehydrated in 200 μL of water at 37°C for 30 min. Next, 75 μL of solubilized CPS samples were added to 25 μL of 4× SDS-gel loading dye, and then 20 μL of each sample was resolved on a 4%–15% Mini-PROTEAN TGX stain-free pre-cast gel (Bio-Rad) by applying 300 V for 4.5 h at 4°C. ..

    Article Title: Resistance exercise enhances long-term mTORC1 sensitivity to leucine
    Article Snippet: .. Protein Transfer Samples were prepared 3:4 with laemmli buffer containing 10% 2-mercaptoethanol (Bio-rad laboratories) and heated at 95° for 5 min. Proteins were run on a 4–20% Mini-PROTEAN TGX Stain Free Pre-Cast Gel (Biorad Laboratories) for 45 min at 120–140 V. After electrophoresis, a picture of the gel was taken under UV-light to determine protein loading using strain-free technology. .. Proteins were transferred via semi-dry transfer onto a polyvinylidene fluoride membrane (Bio-rad, 170-4156) and subsequently blocked for 1 h at room temperature with 5% milk in TBS-Tween.

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    Article Title: Connections between Klebsiella pneumoniae Bloodstream Dynamics and Serotype-Independent Capsule Properties
    Article Snippet: .. Next, 75 μL of solubilized CPS samples were added to 25 μL of 4x SDS-gel loading dye, then 20 μL of each sample was resolved on a 4-15% Mini-PROTEAN TGX stain-free pre-cast gel (Bio-Rad) by applying 300 V for 4.5 hours at 4°C. ..

    Article Title: Arginine Regulates the Mucoid Phenotype of Hypervirulent Klebsiella pneumoniae
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    Incubation:

    Article Title: Connections between Klebsiella pneumoniae bloodstream dynamics and serotype-independent capsule properties.
    Article Snippet: .. Solutions were incubated on ice for 20 min and then pelleted at 17,000 × g for 5 min. Pellets were rehydrated in 200 μL of water at 37°C for 30 min. Next, 75 μL of solubilized CPS samples were added to 25 μL of 4× SDS-gel loading dye, and then 20 μL of each sample was resolved on a 4%–15% Mini-PROTEAN TGX stain-free pre-cast gel (Bio-Rad) by applying 300 V for 4.5 h at 4°C. ..

    Article Title: Connections between Klebsiella pneumoniae bloodstream dynamics and serotype-independent capsule properties
    Article Snippet: .. Solutions were incubated on ice for 20 min and then pelleted at 17,000 × g for 5 min. Pellets were rehydrated in 200 μL of water at 37°C for 30 min. Next, 75 μL of solubilized CPS samples were added to 25 μL of 4× SDS-gel loading dye, and then 20 μL of each sample was resolved on a 4%–15% Mini-PROTEAN TGX stain-free pre-cast gel (Bio-Rad) by applying 300 V for 4.5 h at 4°C. ..

    Electrophoresis:

    Article Title: Resistance exercise enhances long-term mTORC1 sensitivity to leucine
    Article Snippet: .. Protein Transfer Samples were prepared 3:4 with laemmli buffer containing 10% 2-mercaptoethanol (Bio-rad laboratories) and heated at 95° for 5 min. Proteins were run on a 4–20% Mini-PROTEAN TGX Stain Free Pre-Cast Gel (Biorad Laboratories) for 45 min at 120–140 V. After electrophoresis, a picture of the gel was taken under UV-light to determine protein loading using strain-free technology. .. Proteins were transferred via semi-dry transfer onto a polyvinylidene fluoride membrane (Bio-rad, 170-4156) and subsequently blocked for 1 h at room temperature with 5% milk in TBS-Tween.



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    Analysis of SP-EVs fractions by (a) transmission electron microscopy (TEM) and (b, c) nanoparticle tracking analysis (NTA, n = 4). (d) Protein concentration (μg/μL) (n = 9) was assessed by Abs280 nm. <t>(e)</t> <t>SDS-PAGE</t> (15%) protein profile of SP-EV preparations and SP (60 μg protein/lane) (n = 3) stained with Coomassie brilliant blue. (f) Western blot analysis (60 μg protein/lane) of EV preparations and complete SP using antibodies against EV-associated proteins CD9, CD63, and HSP70, endoplasmic reticulum marker calnexin (CNX), and a non-vesicular extracellular particle apolipoprotein C-III (ApoC3) (n = 3) (full western blot images in Supplementary Figure 2). SP: seminal plasma; P1–2: pool of fractions in size-exclusion chromatography; CL: cell lysate; Mw: molecular weight marker. Different letters (a, b) above the bars indicate statistically significant differences (p < 0.05) between methods (C, P1, and P2), as determined by a linear mixed-effects model and Tukey-adjusted pairwise comparisons. A significant positive correlation was observed between particle concentration and protein concentration across samples (Spearman’s ρ = 0.747, p = 0.0009). All data are expressed as mean ± SD of four independent experiments (n = 4). Scale bar = 200 nm.
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    Image Search Results


    Analysis of SP-EVs fractions by (a) transmission electron microscopy (TEM) and (b, c) nanoparticle tracking analysis (NTA, n = 4). (d) Protein concentration (μg/μL) (n = 9) was assessed by Abs280 nm. (e) SDS-PAGE (15%) protein profile of SP-EV preparations and SP (60 μg protein/lane) (n = 3) stained with Coomassie brilliant blue. (f) Western blot analysis (60 μg protein/lane) of EV preparations and complete SP using antibodies against EV-associated proteins CD9, CD63, and HSP70, endoplasmic reticulum marker calnexin (CNX), and a non-vesicular extracellular particle apolipoprotein C-III (ApoC3) (n = 3) (full western blot images in Supplementary Figure 2). SP: seminal plasma; P1–2: pool of fractions in size-exclusion chromatography; CL: cell lysate; Mw: molecular weight marker. Different letters (a, b) above the bars indicate statistically significant differences (p < 0.05) between methods (C, P1, and P2), as determined by a linear mixed-effects model and Tukey-adjusted pairwise comparisons. A significant positive correlation was observed between particle concentration and protein concentration across samples (Spearman’s ρ = 0.747, p = 0.0009). All data are expressed as mean ± SD of four independent experiments (n = 4). Scale bar = 200 nm.

    Journal: Bio-protocol

    Article Title: Uptake Assay of Ram Seminal Plasma Extracellular Vesicles to Sperm

    doi: 10.21769/BioProtoc.5653

    Figure Lengend Snippet: Analysis of SP-EVs fractions by (a) transmission electron microscopy (TEM) and (b, c) nanoparticle tracking analysis (NTA, n = 4). (d) Protein concentration (μg/μL) (n = 9) was assessed by Abs280 nm. (e) SDS-PAGE (15%) protein profile of SP-EV preparations and SP (60 μg protein/lane) (n = 3) stained with Coomassie brilliant blue. (f) Western blot analysis (60 μg protein/lane) of EV preparations and complete SP using antibodies against EV-associated proteins CD9, CD63, and HSP70, endoplasmic reticulum marker calnexin (CNX), and a non-vesicular extracellular particle apolipoprotein C-III (ApoC3) (n = 3) (full western blot images in Supplementary Figure 2). SP: seminal plasma; P1–2: pool of fractions in size-exclusion chromatography; CL: cell lysate; Mw: molecular weight marker. Different letters (a, b) above the bars indicate statistically significant differences (p < 0.05) between methods (C, P1, and P2), as determined by a linear mixed-effects model and Tukey-adjusted pairwise comparisons. A significant positive correlation was observed between particle concentration and protein concentration across samples (Spearman’s ρ = 0.747, p = 0.0009). All data are expressed as mean ± SD of four independent experiments (n = 4). Scale bar = 200 nm.

    Article Snippet: 10% and 15% SDS-PAGE pre-cast gels (Bio-Rad, catalog number: 456-8033) 8.

    Techniques: Transmission Assay, Electron Microscopy, Protein Concentration, SDS Page, Staining, Western Blot, Marker, Clinical Proteomics, Size-exclusion Chromatography, Molecular Weight, Concentration Assay